Table 2.

N-terminal domain interactions with Ure2p

Plasmid	URE2 part	GFP signal*	Curing,† USA−/total	Interference,‡ USA+/106
pH199	–GFP	+ cytopl.	15/718	13
pVTG4	M1, M2–R65–GFP	+	439/440	68,000
pH545	M1, N3–R65–GFP	+	200/200	93,000
pH486	M1, N4–R65–GFP	+	100/100	73,000
pH762	M1, N5–R65–GFP	+	100/100	22,000
pH487	M1, G6–R65–GFP	+	9/300	11,000
pH408	M1, N7–R65–GFP	+	229/340	109,000
pH763	M1, V9–R65–GFP	+	197/200	35,000
pH764	M1, N11–R65–GFP	+	6/180	79,000
pH349	M1, S13–R65–GFP	+	0/118	71
pH350	M1, R24–R65–GFP	+ cytopl.	1/118	3
pH351	M1, S34–R65–GFP	+	0/118	8
pH352	M1, N45–R65–GFP	+ cytopl.	0/118	3
pH769	M1–S33, S63–R65–GFP	+ cytopl.	5/100	4
pH768	M1–I35, S63–R65–GFP	+ cytopl.	4/100	1
pH767	M1–F37, S63–R65–GFP	+ mainly cytopl.	4/100	4,600
pH442	M1–F39, S63–R65–GFP	+	0/240	130,000
pH484	M1–V43, S63–R65–GFP	−	0/200	66
pH548	M1–N44, S63–R65–GFP	+	129/300	60,000
pH547	M1–N45, S63–R65–GFP	+	101/200	57,000
pH546	M1–N46, S63–R65–GFP	+	80/200	61,000
pH485	M1–N47, S63–R65–GFP	+	295/300	39,000
pH766	M1–N49, S63–R65–GFP	+	86/100	70,000
pH441	M1–N50, S63–R65–GFP	+	139/140	63,000
pH765	M1–S53, S63–R65–GFP	+	95/100	53,000

^*The GFP signal of most fusion constructs transformed into the [URE3] strain was aggregated. Others showed an even cytoplasmic distribution (‘cytopl.’). 

^† Curing was tested as in Table 1. 

^‡ Interference was measured as USA+ cells per 10⁶ cells. All USA+ clones tested became USA− on loss of the plasmid, indicating that this is not [URE3] induction, but simply interference with Ure2p action.