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TTR fibril formation time courses detected by turbidity at 400 nm (OD400). (a) Amyloid fibril formation mediated by partial acid denaturation of TTR (0.20 mg/ml) in acetate buffer (pH 4.4, 37°C, Fig. 2 symbols apply). (b) TTR (0.10 mg/ml) amyloid fibril formation enabled by MeOH-induced denaturation [50% (vol/vol) in Tris buffer (pH 7.0, 25°C)].
