. 2002 Dec 10;99(Suppl 4):16462–16469. doi: 10.1073/pnas.162371699

Table 1.

HP1 interacting partners and candidate partners

Protein	Organism	HP1 variant	Methodology	HP1 domain	Ref(s).
Transcription regulation/chromatin modifying proteins
H1	Drosophila	HP1	rPD	nd	46
HP1-BP74 H1-like	Mouse	mHP1α	Y2H, FW, rPD	Hinge region	46, 86
H3	Mouse	mHP1α, M31, M32	FW, rPD, exIP	CD	46, 54
H3	Drosophila	HP1	rPD	nd	46
Methylated K9 of H3	S. pombe	Swi6	rPD, ChIP	CD	38, 43
Methylated K9 of H3	Drosophila	HP1	IF, FAITC, NMR	CD	26, 41
Methylated K9 of H3	Mouse	mHP1α, M31, M32	rPD	CD	39
Methylated K9 of H3	Human	HP1^Hsα, HP1^Hsβ, HP1^Hsγ	rPD, SPRA	CD	38
H4	Mouse	M31	rPD	nd	54
H4	Drosophila	HP1	In vitro cross-linking	CSD	34
MacroH2A1.2^*	Mouse	M31	IF	nd	87
SUVAR3–9	Drosophila	HP1	IF, Y2H, exIP	CSD	42
Suv39h1	Mouse	M31	IF, exIP, SED	nd	88, 89
SUV39H1	Human	HP1^Hsβ	IF, exIP, SED	nd	88, 89
Suvar3–7	Drosophila	HP1	IF, Y2H, exIP	CSD	35, 50
KAP-1/TIF1β	Human	HP1^Hsα, HP1^Hsγ	IF, rPD, exIP, SPRA, GFC	CSD	28, 46–48
KAP-1/TIF1β	Mouse	mHP1α, M31, M32	IF, rPD, Y2H, exIP, GFC	CSD	28, 37, 47, 86, 90
TRF1/PIN2	Mouse	M31	IF	nd	91
TAF_II130	Human	HP1^Hsα, HP1^Hsγ	Y2H, transPD, exPD	CSD	36
TIF1α	Mouse	mHP1α, M31, M32	Y2H, rPD	CSD	86, 90
mSNF2β	Mouse	mHP1α	Y2H	CSD	86
Rb	Human	HP1, HP1^Hsγ	Y2H, exPD, exIP, ChIP	nd	57, 82
Rb	Maize	HP1^γ	rPD, Y2H	nd	82
Dnmt3a	Mouse cells	mHP1α	IF	nd	92
Dnmt3b	Mouse cells	mHP1α	IF	nd	92
ATRX/HP1-BP38	Mouse	mHP1α, M31	Y2H, IF	CSD	86, 93
Pim-1	Human	HP1^Hsγ	Y2H, exIP, rPD	CSD	65
CKII	Drosophila	HP1	In vitro phosphorylation	nd	63
dAF10	Drosophila	HP1	transPD	CSD	94
DNA replication and repair
CAF-1 p150	Mouse	mHP1α, M31	IF, Y2H, rPD, GFC, NMR	CSD	28, 37
CAF-1 p150	Human	HP1^Hsα	rPD	CSD	48
Ku70	Human	HP1^Hsα	Y2H, rPD, exIP	CSD	95
BRCA-1^*	Human	HP1^Hsα	IF	nd	96
ORC1	Drosophila	HP1	transIP	CD, CSD	58
ORC2	Drosophila	HP1	IF, exPD, exIP, transIP	CD, CSD	58
ORC3	Drosophila	HP1	transIP	CD, CSD	58
ORC4	Drosophila	HP1	transIP	CD, CSD	58
ORC5	Drosophila	HP1	exIP, transIP	CD, CSD	58
ORC6	Drosophila	HP1	exIP, transIP	CD, CSD	58
Xorc1	Xenopus	XHP1^α, XHP1^γ	Y2H	nd	58
HOAP	Drosophila	HP1	IF, exIP	nd	62
Nuclear architecture
Lamin B receptor	Human	HP1^Hsα, HP1^Hsβ, HP1^Hsγ	Y2H, rPD, exPD, transPD, exIP	CSD	48, 52–54
HP1-BP84	Mouse	mHP1α, M31	Y2H	CSD	86
Lamin B	Mouse	M31	BA	CD	55
LAP2β	Mouse	M31	BA	CD	55
Nuclear envelope	Mouse	mHP1α, M31, M32	IF, BA	CD	55
Other chromosome-associated proteins
Psc3	S. pombe	Swi6	IF, Y2H, exPD, ChIP	CD+Glu-rich	13
DDP1	Drosophila	HP1	IF	nd	97
Arp4/dArp6	Drosophila	HP1	IF	nd	98, 99
INCENP	Human	HP1^Hsα, HP1^Hsγ	Y2H, transPD	Hinge region	100
Ki-67	Human	mHP1α, M31, M32	Y2H, exPD, IF	CSD	101
SP100B	Human	HP1^Hsα, HP1^Hsβ, HP1^Hsγ	IF, Y2H, rPD, transPD	CSD	48, 102, 103
EST AA153281	Mouse	mHP1α, M31	Y2H, rPD	CSD	37
EST AA003533	Mouse	mHP1α, M31	Y2H, rPD	CSD	37

BA, binding assay; ChIP, chromatin immunoprecipitation; exIP, co-immunoprecipitation using extract; exPD, pull-down assay using extracts; FAITC, fluorescence anisotropy, isothermal titration calorimetry; FW, far Western analysis; GFC, gel filtration chromatography; IF, immunofluorescence colocalization; nd, not determined; rIP, coprecipitation using recombinant proteins; rPD, pull-down assay using recombinant proteins; transIP, immunoprecipitation with in vitro-translated protein; transPD, pull-down assay using in vitro translated protein; SED, sedimentation assay; SPRA, surface plasmon resonance analysis; Y2H, yeast two-hybrid assay.

Denotes cell cycle-dependent association.