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. 2002 Dec 10;99(Suppl 4):16491–16498. doi: 10.1073/pnas.162371599

Figure 2.

Figure 2

Southern blot analysis of FWA and MEA-ISR. (A Upper) A diagram of the CfoI and BglII restriction fragments present in the FWA gene. The inner two CfoI sites and the BglII site are within the methylated direct repeats of the FWA promoter, shown as arrows above. The region analyzed by bisulfite sequencing is also noted. (Lower) DNA blots of equivalent amounts of CfoI (Left) and BglII (Right) digested genomic DNA probed with a 1.74-kb fragment corresponding to the diagram on top. The positions of the methylated (M), unmethylated (U), and partially methylated (P) bands are shown, as well as the size of the bands in kilobases. drm1 drm2 cmt3-7 A and B are two independently isolated triple mutant strains. (B Upper) A diagram of the HpaII/MspI restriction fragments present in the MEA-ISR region. The inner HpaII/MspI site lies in the first of seven direct repeats (shown as arrows) and the probe lies in the unique sequence between these repeats and the MEA locus. (Lower) DNA blots of equivalent amounts of HpaII (Left) and MspI (Right) digested genomic DNA probed with a 1.1-kb fragment corresponding to the diagram on top.