Figure 1.

PR-1, GST1, and PDF1.2 Expression and Systemic Spread of TCV in Di-17 × Col-0 F₁ and F₂ Plants.

Expression of PR-1, GST1, and PDF1.2 in the inoculated leaves of TCV (T)- and mock (M)-infected Di-17 and Col-0 plants and the F₁ and F₂ progeny obtained from a cross between them. The inoculated leaves were monitored for the presence (+) or absence (−) of the HR, and the RNA was extracted from the inoculated leaves at 4 DPI. The replication and spread of the virus were analyzed by extracting RNA from the uninoculated (TCV-U) bolt tissue at 10 DPI. Two F₁ and five F₂ plants were analyzed. The genotype of the F₂ plants at the HRT locus was determined by CAPS analysis; both homozygous and heterozygous plants were analyzed for the expression of defense genes in inoculated leaves, the presence of TCV RNA in the uninoculated bolt tissue, and the appearance (S) or absence (R) of disease symptoms. The blot (1 to 11) was sequentially probed for the indicated genes (PR-1, GST1, and PDF1.2). RNA extracted from the systemic tissues was run on a separate gel; rRNA was used as an internal control for gel loading and transfer for both blots.