Figure 3.

PR-1 Expression and Systemic Spread of TCV in Di-17 × nahG F₁ Plants.

Two F₁ plants each from a cross of Di-17 and NahG Nö and its reciprocal cross were inoculated with TCV and monitored for the presence (+) or absence (−) of the HR. TCV (T)- or mock (M)-inoculated Di-17 parental plants were used as controls. Expression of the PR-1 gene in the inoculated leaves was analyzed at 4 DPI. The systemic spread and replication of the virus (TCV-U) were determined by analyzing RNA extracted from the uninoculated bolt tissues at 10 DPI. The blot (1 to 7) was sequentially probed for PR-1, the nahG transgene, and TCV genome. RNA extracted from the systemic tissues was run on a separate gel. rRNA was used as an internal control for gel loading and transfer for both blots.