Figure 4.

Effects of VIGS on Transcription of CesA Genes.

Shown are agarose gels of RT-PCR products generated from primers for GAPDH mRNA (A) and for NbCesA-1 mRNA (B). Total RNA was isolated from leaves of different, individually infected N. benthamiana plants inoculated with the PVX control or PVX–NtCesA-1a/1b (1a and 1b, respectively) constructs. The PCR products were routinely excised from the gel, and their identities were confirmed by nucleotide sequence analysis to be 100% identical with the NbCesA-1 cDNA fragment. Molecular markers (M) are in the first lane, and the arrowheads indicate the 500-bp band. The right-hand lane (N) is the control RT-PCR reaction, in which no DNA was added. The diffuse band seen in this reaction mixture was generated from the oligonucleotide primers.