Table 2.
Linkage Analysis of Carboxyl-Reduced Cell Wall Preparations from Leaves of N. benthamiana
| Deduced Linkagea | PVX–NtCesA-1a | PVX–NtCesA-1b | PVX–NtCesA-2 | PVX-Control |
|---|---|---|---|---|
| Mol%b,c | ||||
| Glucosyl | ||||
| 4-Glcp | 32.6 ± 3.1d | 32.0 ± 3.9d | 42.6 ± 1.5d | 41.4 ± 1.2d |
| 4,6-Glcp | 3.5 ± 0.5 | 3.4 ± 0.6 | 3.7 ± 0.5 | 3.8 ± 0.5 |
| t-Glcp | 0.9 ± 0.3 | 1.0 ± 0.3 | 0.8 ± 0.1 | 1 .1 ± 0.4 |
| 3,4-Glcp | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.6 ± 0.1 | 0.4 ± 0.1 |
| Galactosyl uronic acid | ||||
| 4-GalpA | 19.2 ± 2.0d | 21.2 ± 2.1d | 10.3 ± 1.1d | 11.3 ± 1.0d |
| 4-GalpA-Ester | 9.2 ± 1.9 | 10.1 ± 3.6 | 10.4 ± 1.5 | 11.1 ± 1.0 |
| t-GalpA | 0.6 ± 0.3 | 0.6 ± 0.3 | 0.4 ± 0.1 | 0.3 ± 0.1 |
| t-GalpA-Ester | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.2 | 0.4 ± 0.1 |
| 2,4-GalpA | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.0 | 0.1 ± 0.0 |
| 2,4-GalpA-Ester | 0.1 ± 0.0 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.0 |
| 3,4-GalpA | 0.7 ± 0.2 | 0.8 ± 0.5 | 0.4 ± 0.1 | 0.5 ± 0.1 |
| Xylosyl | ||||
| 4-Xylp | 6.0 ± 0.9 | 5.4 ± 0.6 | 5.5 ± 0.6 | 5.6 ± 0.7 |
| t-Xylp | 1.7 ± 0.2 | 1.9 ± 0.2 | 1.8 ± 0.2 | 1.7 ± 0.2 |
| 2-Xylp | 1.0 ± 0.1 | 1.0 ± 0.2 | 1.1 ± 0.1 | 1.1 ± 0.2 |
| 2,4-Xylp | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.1 |
| Galactosyl | ||||
| 4-Galp | 3.6 ± 0.4 | 3.0 ± 0.4 | 2.4 ± 0.3 | 3.4 ± 0.6 |
| t-Galp | 3.0 ± 0.2 | 2.7 ± 0.0 | 1.9 ± 0.1 | 2.3 ± 0.2 |
| 4,6-Galp | 0.9 ± 0.2 | 1.2 ± 0.3 | 0.9 ± 0.1 | 0.7 ± 0.1 |
| 6-Galp | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.1 |
| 2,4-Galp | 0.4 ± 0.1 | 0.5 ± 0.2 | 0.7 ± 0.0 | 0.5 ± 0.1 |
| Arabinosyl | ||||
| t-Araf | 2.6 ± 0.2 | 2.5 ± 0.3 | 2.2 ± 0.2 | 2.2 ± 0.1 |
| 5-Araf | 2.3 ± 0.3 | 1.4 ± 0.3 | 1.5 ± 0.3 | 1.8 ± 0.3 |
| 3,5-Araf | 0.4 ± 0.1 | 0.4 ± 0.0 | 0.5 ± 0.0 | 0.5 ± 0.1 |
| t-Arap | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 |
| Rhamnosyl | ||||
| 2-Rhap | 2.5 ± 0.6 | 2.7 ± 0.7 | 2.6 ± 0.3 | 2.1 ± 0.4 |
| 2,4-Rhap | 1.7 ± 0.3 | 1.5 ± 0.5 | 1.6 ± 0.2 | 1.5 ± 0.3 |
| t-Rhap | 0.6 ± 0.0 | 0.6 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 |
| Mannosyl | ||||
| 4-Manp | 2.8 ± 0.3 | 2.4 ± 0.0 | 4.4 ± 0.6 | 3.0 ± 0.3 |
| 4,6-Manp | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 |
| Glucosyl uronic acid | ||||
| 4-GlcpA | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 4-GlcpA-Ester | 0.6 ± 0.1 | 0.6 ± 0.1 | 0.8 ± 0.0 | 0.8 ± 0.0 |
| t-GlcpA | 0.4 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.1 | 0.3 ± 0.0 |
| t-GlcpA-Ester | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 |
| Fucosyl | ||||
| t-Fucp | 0.3 ± 0.0 | 0.3 ± 0.1 | 0.3 ± 0.0 | 0.2 ± 0.0 |
a
Deduction of the glycosidic linkages was based on Carpita and Shea (1989) and esterifications on Kim and Carpita (1992).
b
Data represent means ±se of four or five individuals for PVX-GS1b and PVX-GS2, or PVX-GS1a and PVX-control, respectively.
c
The data were calculated without the following list of linkage types, which were found in trace amounts: 3-Glcp; 3,4,6-Glcp; 3,4-GalpA-Ester; 3,4-Xylp; 2-Galp; 3-Galp; 3,4-Galp; 3,6-Galp; 2,3,4-Galp; 3,4,6-Galp; 2-Araf; 3-Araf; 2-Arap; 2,5-Araf; 2,3-Rhap; t-Manp; and 3,4,6-Manp.
d
Values showing significant alteration in response to infection, as discussed in the text.