Figure 4.

Effect of Wounding or Systemin on Expression of Pti4, Pti5, Pti6, Pin2, and GluB.

Fully expanded leaves from 4-week-old tomato plants were used for analysis of gene expression. Equal loading was verified by visualizing the rRNA in the RNA gels stained with ethidium bromide. Pin II, Pin2.

(A) Tomato RG-PtoR plants were wounded by rubbing leaves with carborundum, after which total RNAs were isolated from leaves at the times indicated. Duplicate RNA gel blots were hybridized with radiolabeled Pti4, Pti5, Pti6, Pin2, or GluB probes. Note that lane C for Pti5 contains total RNA isolated from leaves of 8-week-old tomato plants in which Pti5 transcript is detectable.

(B) Tomato RG-PtoR plants were treated with 1 nM systemin, after which total RNAs were isolated from leaves at the times indicated. Duplicated RNA gel blots were hybridized with radiolabeled Pti4 or Pin2 probes.