Figure 1.

Purification of Elicitor-Active Tetraglucosyl Glucitols by HPLC.

Fragments of the β-glucan generated by the enzymatic digestion were fractionated on Biogel P-4. All fractions larger than tetramers showed a potent elicitor activity. The pentaose fraction was reduced by NaBH₄, and 5 mg of the reduced product was further fractionated by reverse-phase HPLC on an Inertsil ODS 3 column. The column was equilibrated with 8% CH₃OH at 0.5 mL/min for 2 hr before the addition of the sample and was eluted with the same buffer. Peaks indicated by the arrows showed detectable elicitor activity.