Figure 4.

Ca²⁺-dependence of IQ-Alexa-calmodulin interaction Binding of IQ peptides to Alexa-calmodulin (50 nM) in 100 μM EGTA or 25 μM CaCl₂ as described for Fig. 3; error bars indicate standard deviation with n = 6. Data were fit with a modified Hill equation (equation (4)).