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. 2002 Nov 26;3:31. doi: 10.1186/1471-2091-3-31

Table 2.

Interaction of Myo1c IQ peptides with unlabeled calmodulin

Peptide
 Apparent Kd,
EGTA
 Apparent Kd,
CaCl2
IQ1 4.0 ± 0.8 μM 56 ± 13 μM
IQ2 4.2 ± 2.0 μM 70 ± 15 μM
IQ3 1.7 ± 0.7 μM 3.2 ± 1.1 μM

Quenching of Alexa-calmodulin fluorescence was used to estimate binding affinity of IQ peptides to calmodulin using equation (7). Values reported are means ± standard error (n = 3 to 4 independent experiments for each value). IQ4 affinities were not accurately measured under conditions of this assay, but were >100 μM.