Table 5.
Hydrodynamic analysis of T701-Myo1c in the presence of 5 μM calmodulin
|
Property |
Temperature |
T701-Myo1c 100 μM EGTA |
T701-Myo1c 25 μM CaCl2 |
| Sedimentation coefficient | 25°C | 4.68 ± 0.11 S (n = 2) | 3.95 ± 0.33 S (n = 4) |
| Molecular mass | 25°C | 92 ± 4 kD | 62 ± 12 kD |
| Calmodulins per molecule | 25°C | 2.8 ± 0.2 | 1.0 ± 0.7 |
Purified T701-Myo1c co-expressed with calmodulin, was subjected to sucrose-gradient centrifugation in the presence of 5 μM calmodulin in either 100 μM EGTA or 25 μM CaCl2. T701-Myo1c was detected by ELISA. We estimated molecular mass and hence stoichiometry by assuming that Stokes radii of the complexes were unchanged by the presence of calmodulin, an assumption that might have led to an underestimate of the mass. Values reported are mean ± range or standard deviation.