Table 2.
Comparison of microarray, quantitative Northern slot blot and in situ hybridization data for genes validated in this study.
| Array Data | Northern Data | In situ | Known | ||||||||
| Descriptions | Accession Number | Naïve Mean intensity ± S.D. | Axotomy Mean intensity ± S.D. | Fold change | P value | Fold change | P value | Regulation | Regulation | ||
| GTP cyclohydrolase I | M58364 | 222 ± 12 | 1605 ± 194 | 7.2 | 0.0064 | ** | 3.8 | 0.0000 | *** | ↑ | |
| Endothelin-1 | M64711 | 538 ± 170 | 716 ± 34 | 1.3 | 0.2076 | 1.7 | 0.0004 | *** | |||
| Cannabinoid CB1 receptor | X55812 | 576 ± 21 | 388 ± 49 | -1.5 | 0.0118 | * | -1.2 | 0.4221 | |||
| Cholecystokinin-B receptor | M99418 | 588 ± 145 | 534 ± 56 | -1.1 | 0.6011 | 2.7 | 0.0008 | *** | ↑(31,32) | ||
| 53 kD polypeptide | X02601 | 645 ± 194 | 723 ± 113 | 1.1 | 0.5875 | 2.4 | 0.0433 | * | |||
| Small proline-rich protein 1a (EST195714) | AA891911 | 1063 ± 205 | 3856 ± 263 | 3.6 | 0.0002 | *** | 3.3 | 0.0267 | * | ↑(8) | |
| ET-B endothelin receptor | X57764 | 1228 ± 1015 | 1448 ± 1131 | 1.2 | 0.8145 | 1.2 | 0.1445 | ||||
| Guanine nucleotide-releasing protein (MSS4) | L10336 | 1301 ± 335 | 1286 ± 174 | 1.0 | 0.9478 | -1.1 | 0.0562 | ||||
| Immediate-early serum-responsive JE (IES-JE) | X17053 | 1539 ± 473 | 3864 ± 818 | 2.5 | 0.0208 | * | 2.0 | 0.0089 | ** | ↑ | |
| Peripheral-type benzodiazepine receptor | J05122 | 2664 ± 314 | 4148 ± 459 | 1.6 | 0.0132 | * | 2.1 | 0.0078 | ** | ||
| 5HT-3 receptor | U59672 | 2750 ± 254 | 1413 ± 276 | -1.9 | 0.0036 | ** | -2.6 | 0.0140 | * | ↓ | |
| Gadd45 | L32591 | 3394 ± 195 | 14,021 ± 644 | 4.1 | 0.0005 | *** | 5.9 | 0.0047 | ** | ||
| Pituitary adenylate cyclase activating peptide(PACAP) | X80290 | 3466 ± 434 | 12,182 ± 290 | 3.5 | 0.0000 | *** | 4.6 | 0.0004 | *** | ↑(6,55) | |
| Phopholemman chloride channel (EST189142) | AA799645 | 3629 ± 327 | 8094 ± 283 | 2.2 | 0.0001 | *** | 1.6 | 0.0062 | ** | ||
| VGF (nerve growth factor-inducible protein) | M74223 | 3791 ± 619 | 10,200 ± 748 | 2.7 | 0.0004 | *** | 2.7 | 0.0050 | ** | ↑ | |
| HNF-3/fork-head homolog-2 (HFH-2) | L13202 | 3961 ± 396 | 3640 ± 202 | -1.1 | 0.2994 | 1.3 | 0.0986 | ||||
| GFRα1(RET ligand 1) | U97142 | 4132 ± 755 | 8443 ± 581 | 2.0 | 0.0019 | ** | 2.4 | 0.0029 | ** | ↑(45) | |
| CLP36 (Elfin) | U23769 | 5124 ± 532 | 11,527 ± 666 | 2.2 | 0.0003 | *** | 2.5 | 0.0019 | ** | ||
| Metallothionein-1 (EST211851) | AI102562 | 5628 ± 779 | 6715 ± 319 | 1.2 | 0.1230 | 1.6 | 0.0321 | * | |||
| Calcium channel α-2 subunit (CCHL2A) | M86621 | 7267 ± 1792 | 25,005 ± 1485 | 3.4 | 0.0002 | *** | 4.7 | 0.0125 | * | ↑ | ↑(65) |
| α-2-macroglobulin | M23566 | 8995 ± 1046 | 21,057 ± 3221 | 2.3 | 0.0157 | * | 2.6 | 0.0023 | ** | ||
| Lysozyme (EST196578) | AA892775 | 10,702 ± 3948 | 26,572 ± 2127 | 2.5 | 0.0082 | ** | 2.9 | 0.0024 | ** | ||
| Guanine nucleotide-binding protein G-i, α subunit | M12672 | 10,728 ± 1325 | 10,781 ± 1099 | 1.0 | 0.9608 | 1.1 | 0.0217 | * | |||
| SNAP-25A | AB003991 | 22,532 ± 3763 | 16,441 ± 1731 | -1.4 | 0.0898 | -1.4 | 0.0486 | * | ↓ | ||
Key to abbreviations, * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed unpaired t-test). Down regulated genes are expressed as negative fold changes.