Table 1.
Overview of Pmps, antibodies and immunoblot reactions
| pAb | Pmp | Cl. | Fam. | L | aa | cloned | MS | G | M | IMB | MeOH | Form. |
| 204 | 1 | I | G | I | 923 | 26–923 | - | - | I | 1?2 6 78 10 13 14 20 21 | X | - |
| 207 | 2 | I | G | I | 842 | 20–842 | X | S | M | 2 6 8 10 | X | - |
| - | 3* | I | G | I | ||||||||
| - | 4* | I | G | I | ||||||||
| - | 5* | I | G | I | ||||||||
| 221 | 6 | II | G | I | 1408 | 18–905 | N+C | S | M | 6 8 10X | X | X |
| 220 | 7 | II | G | I | 937 | 25–545 | X | S | 2 6 7 8 10 11 13 | X | - | |
| 201 | 8 | II | G | I | 931 | 21–931 | X | S | M | 8 10 | X | X |
| 208 | 9 | II | G | I | 929 | 27–929 | - | S | I | 2 6 7 8 9? 10 11 13 14 20 | X | u |
| 203 | 10 | II | G | II | 929 | 20–929 | X | - | M | 10 | X | X |
| 195 | 11 | II | G | II | 929 | 1–929 | X | W | M | 6 11 | X | X |
| - | 12# | II | - | I | ||||||||
| 222 | 13 | II | G | I | 974 | 20–542 | X | S | M | not performed | X | - |
| 228 | 14 | II | H | I | 979 | 25–492 | X | S | M | not performed | X | - |
| 229 | 15 | III | EF | I | 939 | 18–478 | - | W | I | - | - | - |
| 230 | 16 | III | EF | N | 935 | 1–472 | - | S | M | 8 10 | - | - |
| - | 17* | III | EF | I | ||||||||
| 231 | 18 | III | EF | N | 893 | 1–566 | - | P | 8 10 | - | - | |
| 234 | 19 | IV | A | I | 948 | 22–501 | - | - | - | - | - | |
| 236 | 20 | IV | BC | I | 1724 | 22–1272 | X | - | M | 20 13 | - | - |
| 237 | 21 | D | I | 1610 | 52–1129 | N+C | - | M | 6 13Y | X | X |
pAb: Antibody number; Pmp: Pmp number: *, genes containing a premature stop; #, naturally short gene; shading indicates either * or #. Cl.: Cluster in the C. pneumoniae genome.L: Predicted leader sequences: I, signal peptidase I cleavage site; II, signal peptidase II cleavage site; -, no predicted cleavage site. Fam.: Closest related C. trachomatis Pmp. aa: Amino acids in full-length Pmp. cloned: First and last amino acid in the recombinant protein. MS: Mass spectrometry identification by: X, peptides dispersed along the entire sequence; N, peptides localized to the N-terminal part; C, peptides localized to the C-terminal part; -, not identified by MS. G: Detection in 1D IMB by Grimwood et al.: S, strong reaction; W, weak reaction; P, possible reaction; -, no reaction. M: Detection by Montigiani et al. [24]: M, MS and immunoblot identification; I, immunoblot detection alone. IMB: Immunoblotting results: bold numbers indicate strong reaction; X is a C-terminal fragment of Pmp6; Y is an N-terminal fragment of Pmp21; results marked ? were inconclusive. MeOH: MeOH fixation IMF at 72 hpi: X, reaction; -, no reaction. Form.: Formalin fixation IMF at 72 hpi: X specific reaction; u, unspecific reaction; -, no reaction.