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Titration of E. coli template DNA for 16S rDNA PCR assay, using 515F and RD1 primers. Lanes: L, 100-bp DNA ladder; 1, 14 ng; 2, 1.4 ng; 3, 0.14 ng; 4, 1.4 × 10-2 ng; 5, 1.4 × 10-3 ng; 6, 1.4 × 10-4 ng; 7, 1.4 × 10-5 ng; 8, 1.4 × 10-6 ng; 9, 1.4 × 10-7 ng; 10, 1.4 × 10-8 ng; 11, 1.4 × 10-9 ng; 12, Negative control (sterile distilled water); 13, Positive control (purified E. coli DNA).
