Skip to main content
. 2002 Dec 9;3:34. doi: 10.1186/1471-2164-3-34

Table 2.

PCR assay results using primer sets designed for pXO2 ORF sequences.

ORF Nucleotides Gene ID or similarity S2-8 3A DC-17 ALH konku HD571 F1-15 4342 43881 33679 AWO6
5 2240–3088 unidentified X
7 3449–4057 unidentified
9 5227–7158 sim. trsE, S. aureus X X
10 7178–7846 unidentified X
14 8704–11562 c.h.p. C. perfringens X
15 11588–12379 c.h.p. C. perfringens
16 12381–14216 sim. trsK, L. lactis X
17 14265–16145 unidentified
24 18442–18942 unidentified
25 18975–20306 sim. pX01 ORF-59, B. anthracis X
28 21387–22628 unidentified X X
29 22897–24546 unidentified
30 24561–25625 unidentified
32 26752–27000 unidentified X X
33 27515–28045 unidentified X X
35 29882–30571 sim. rep63A, AWO6 pAW63
37 31610–32386 sim. pAW63 X
38 32577–34115 sim. repS, AWO6 pAW63 X X
39 35021–35887 sim. repB, AWO6 pAW63
42 37951–39510 sim. S-layer precursor, B. anthracis
44 40988–41308 unidentified
45 41900–42211 conserved domain, several bacteria
46 42260–42925 CAAX amino term. protease family X X X
47 43636–44100 c.h.p. several bacteria X X
48 44477–45067 transcriptional repressor, tetR fam. X X X X X
49 45891–46361 IS231
50 46400–46891 IS231
51 47474–47641 sim. bacitracin
53 49418–50866 sim. to acpA, B. anthracis
55 52795–54195 dep
56 54378–55612 capA
57 55625–56074 capC
58 56089–57483 capB
59 60856–61407 signal peptide
60 61759–62496 unidentified
61 62841–63251 sim. pX01 atxA, B. anthracis
64 68909–70360 acpA
66 73500–75059 traC X X
68 76097–76690 unidentified X X
69 76918–78183 uvx X
71 79219–80772 unidentified
73 82311–83936 repressor
74 85420–85857 unidentified
76 86664–87491 topoisomerase X X
77 87888–88688 unidentified
80 90752–91735 unidentified
81 91802–93571 unidentified X
Column Totals 0 1 1 1 2 0 2 0 1 10 16

Bacterial isolates designations are abbreviated as follows: S2-8, B. cereus S2-8; 3A, B. cereus 3A; DC-17, B. cereus DC-17; ALH, B. thuringiensis Al-Hakam; konku, B. thuringiensis subsp. konkukian; HD571, B. cereus (HRRL HD-571; F1-15, B. cereus F1-15; 4342, B. cereusATCC 4342; 43881, B. cereus ATCC 43881; 33679, B. thuringiensis ATCC 33679; AWO6, B. thuringiensis AWO6. See Table 1 for source and Jaccard distances of AFLP profiles. 'sim.' = similar to. 'c.h.p.' = conserved hypothetical protein.