Figure 2.

Anomalous electrophoretic mobilities of short unphosphorylated oligonucleotides. The oligos shown in Figure 1A were labeled with ³²P either by addition of a single labeled dGMP residue to the 3′ terminus with terminal transferase (TT oligos; odd lanes), or by addition of ³²PO₄ to the 5′ terminus with polynucleotide kinase (K oligos; even lanes). The labeled oligos were electrophoresed in a denaturing gel and the bands were detected by exposure to a phosphorimager screen and subsequent analysis in a phosphorimager apparatus.