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. 2002 Dec 15;30(24):5570–5578. doi: 10.1093/nar/gkf676

Figure 5.

Figure 5

A scheme showing the minimal lengths required for primers aligned in the beginning, middle and next to the end of the template to be extended with the Tetrahymena telomerase. A segment of the Tetrahymena TER subunit is shown here (6). This segment includes the RNA template region, nucleotides 43–51. Nucleotides 43–49 serve as templating residues, i.e. they are copied by the telomerase into DNA telomeric repeats. Nucleotides 50–51 serve to align the primers along the template, but are not copied by the enzyme (36). The DNA nucleotide sequences indicated in schemes I, II and III designate the shortest primers that could be extended with the telomerase either by a single nucleotide, or by multiple repeats, at a primer concentration of 2.5 µM. The underlined sequences designate the shortest primers that could be extended with the telomerase by a single nucleotide at a primer concentration of 50 µM. The rectangle denotes the hypothetical PAS defined in the Discussion.