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. Author manuscript; available in PMC: 2006 Mar 13.
Published in final edited form as: J Biol Chem. 2005 Sep 22;280(46):38328–38336. doi: 10.1074/jbc.M509622200

TABLE I.

Strains used in this work

Strain Relevant characteristics Source or Ref
E. coli
    DH5α F-, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk- mk+), phoA, supE44, λ-thi-1, gyrA96, relA1 (52)
    BL21(DE3) F- ompT hsdSB (rB-mB-) gal dcm (DE3) Novagen
S. cerevisiae
    SDO195 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] (99)
    GHY52 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105] This study
    GHY53 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105-hCTPS1] This study
    GHY54 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105-hCTPS2] This study
    GHY55 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1] This study
    GHY56 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS2] This study