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. Author manuscript; available in PMC: 2006 Mar 13.
Published in final edited form as: J Biol Chem. 2005 Sep 22;280(46):38328–38336. doi: 10.1074/jbc.M509622200

TABLE II.

Plasmids used in this work

Plasmid Relevant characteristics Source or Ref
pET-28b(+) E. coli expression vector with the T7 lac promoter and C-terminal His6-tag fusion Novagen
pET-28b(+)-hCTPS1 CTPS1 derivative of pET-28b(+) This study
pET-28b(+)-hCTPS2 CTPS2 derivative of pET-28b(+) This study
YEpLac181 Multicopy E. coli/yeast shuttle vector with LEU2 (84)
YEpLac195 Multicopy E. coli/yeast shuttle vector with URA3 (84)
pDO105 Derivative of YEpLac181 with the ADH1 promoter and multiple cloning sites (9)
pDO134 URA7 derivative of YEpLac195 (9)
pDO105-hCTPS1 CTPS1 derivative of pDO105 This study
pDO105-hCTPS2 CTPS2 derivative of pDO105 This study