Figure 5.

BAC bins and physical map of the Mi contig. Each bin is defined as a domain in which a set of AFLP markers is shared between BACs. The number of comigrating fragments indicated in the top table is used to estimate the order and size of the overlap. For each shared fragment observed in gel an overlap portion of 3 kb is assumed. The assembly positions of BAC end sequences (orange squares) flanking the T7 (triangle pointing right) and SP6 (triangle pointing left) region on the consensus of P250I2 have been used to calculate actual overlap sizes. BAC end sequences assembled onto P073H07 contigs (horizontal lines) have been used as BAC end sequence tags for extended ordering.