Figure 4.

CAD activities of total proteins and GH2 expression pattern. A, CAD activity and SAD activity of the total proteins from different tissues of the wild-type and gh2 plants at the heading stage. The se bars were obtained from triplicate independent measurements. *, Not detectable. B, Northern-blot analysis of GH2 expression in different tissues of the wild type at the heading stage. Ethidium bromide staining of the 28S rRNA band is shown as the loading control. For both assays, the tissues include panicles, hulls, leaf blades, midribs, sheaths, the first two internodes, and young roots. The leaves eliminated of midrib (blades), midribs, and sheaths were all derived from flag leaves.