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. 2006 Mar;140(3):972–983. doi: 10.1104/pp.105.073007

Figure 5.

Figure 5.

Expression and purification of recombinant GH2 and gh2 proteins in E. coli. Lane 1, The molecular mass ladder; lanes 2, 3, and 4, the crude extracts from induced recombinant E. coli of pGEX6P-1, pGEXGH2, and pGEXgh2; lanes 5 and 6, the purified glutathione S-transferase (GST)-GH2 and GST-gh2 (about 66 kD); lanes 7 and 8, the cleaved fusion proteins GH2 and gh2 (about 40 kD). The arrowheads point to the GST fusion proteins and the cleaved fusion proteins. Proteins were visualized by Coomassie Blue R-250 staining.