Figure 3.

Cd content of wild-type and AtATM3-overexpressing plants. A, Cd content of AtATM3-overexpressing (ATM3-1) and wild-type plants. FW, Fresh weight. B, ¹⁰⁹Cd radioactivity from shoots and roots of Arabidopsis plants treated with 0.1 μm ¹⁰⁹CdCl₂ for 24 h. Arabidopsis plants were grown vertically for 10 d on 0.5× MS agar plates and then treated for 24 h with 100 μm nonradioactive CdCl₂ (A) or 0.1 μm¹⁰⁹CdCl₂ (B). Shoots and roots were harvested separately before measuring Cd content using an atomic absorption spectrometer (A) or liquid scintillation counter (B). See “Materials and Methods” for further details about the experiment. Significant differences from wild type as determined by Student's t test are indicated (*, P < 0.05). Bars = se.