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AtNTF2a and AtNTF2b accumulate at the nuclear envelope. AtNTF2a-GFP (A and B), AtNTF2b-GFP (C and D), and free GFP (E and F) were transiently expressed in tobacco BY-2 protoplasts, and GFP fluorescence was imaged by confocal laser scanning microscopy. A, C, and E, GFP fluorescence; B, D, and F, transmitted light images of protoplasts. Cellular compartments are indicated in E: n, nucleus; no, nucleolus; c, cytoplasm; and v, vacuole. Size bars = 10 μm.
