Table I.
List of plasmids used in this study
Abbreviations: AMPR, ampicillin resistance; Y2H, yeast two hybrid; DBD, DNA-binding domain; AD, activation domain; KanR, kanamycin resistance; SPR, spectinomycin resistance; HYGR, hygromycin resistance.
| Name | Description | Reference |
|---|---|---|
| pAC19 | pGAL 2μ LEU2 AMPR | Schlenstedt et al. (1995) |
| pEG202 (pAC20) | Y2H LexA-DBD fusion plasmid; pADH 2μ HIS3 AMPR | Gyuris et al. (1993) |
| pJG4-5 (pAC21) | Y2H LexA-AD fusion plasmid; pGAL 2μ TRP1 AMPR | Gyuris et al. (1993) |
| pAC626 (pPS883) | Yeast NTF2; CEN URA3 AMPR | Corbett and Silver (1996) |
| pAC370 | Human RAN in pAC21; pGAL 2μ TRP1 AMPR | Clarkson et al. (1997) |
| pAC373 | Yeast RAN (GSP1) in pAC21; pGAL 2μ TRP1 AMPR | Wong et al. (1997) |
| pAC410 | GSP1-GFP (yeast RAN); 2μ URA3 AMPR | Quimby et al. (2001) |
| pAC825 | C-terminal GFP fusion plasmid; pMET CEN URA3 AMPR | Niedenthal et al. (1996) |
| pIM1000/pAC1870 | AtNTF2a in pAC20; pADH 2μ HIS3 AMPR | This study |
| pIM1001/pAC1871 | AtNTF2b in pAC20; pADH 2μ HIS3 AMPR | This study |
| pIM1002/pAC1875 | AtRan1 in pAC21; pGAL 2μ TRP1 AMPR | This study |
| pIM1003/pAC1878 | AtNTF2a in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pIM1004/pAC1879 | AtNTF2b in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pIM1005/pAC1941 | AtNTF2aE38K in pAC20; pADH 2μ HIS3 AMPR | This study |
| pIM1006/pAC1942 | AtNTF2aE91K in pAC20; pADH 2μ HIS3 AMPR | This study |
| pIM1007/pAC1947 | AtNTF2aE38K in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pIM1008/pAC1948 | AtNTF2aE91K in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pIM1009/pAC1949 | AtNTF2a in pAC825; pMET CEN URA3 AMPR | This study |
| pIM1010/pAC1950 | AtNTF2b in pAC825; pMET CEN URA3 AMPR | This study |
| pK7FWG2.0 | C-terminal fusion with GFP; KanR SPR | Karimi et al. (2002) |
| pH2GW7 | Overexpression vector in plant; HYGR SPR | Karimi et al. (2002) |
| pYES2 | Inducible yeast expression vector; pGAL; URA3 AMPR | Invitrogen |
| pIM1011 | AtNTF2a in pENTR TOPO; KanR | This study |
| pIM1012 | AtNTF2b in pENTR TOPO; KanR | This study |
| pIM1013 | AtNTF2aE38K in pENTR TOPO; KanR | This study |
| pIM1014 | AtNTF2a in pK7FWG2.0; KanR SPR | This study |
| pIM1015 | AtNTF2b in pK7FWG2.0; KanR SPR | This study |
| pIM1016 | AtNTF2a in pH2GW7; HYGR SPR | This study |
| pIM1017 | AtNTF2aE38K in pH2GW7; HYGR SPR | This study |
| pIM1018 | AtNTF2a in pYES2; pGAL; URA3 AMPR | This study |
| pIM1019 | AtNTF2b in pYES2; pGAL; URA3 AMPR | This study |
| pIM1020 | AtNTL in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pAC2024 | Yeast NTF2 in pAC19; pGAL 2μ LEU2 AMPR | This study |
| pENTR TOPO | Blunt-end PCR product entry vector; KanR | Invitrogen |