Fig. 1.

Specific RNAs selectively associated with Pum in Drosophila adults and embryos. Rows represent genes (unique DNA elements) and columns represent individual experiments. The color code indicates the degree of enrichment (green–red ratio scale). (A) Relative enrichments of mRNA targets of Pum expressed in adult ovaries. Four experiments with affinity-tagged Pum proteins and three mock experiments are shown. Genes were ordered from top to bottom according to increasing FDRs determined by significance analysis of microarrays (SAM) analysis. Arrowheads depict enrichment of previously known Pum targets. (B) Pum RNA targets selected in embryos. A total of 174 transcripts representing 165 genes were clustered based on Euclidean distances with smd (50). (C) Diagram showing overlap of adult and embryo Pum associated mRNAs.