Alignment of predicted mouse (strain C57BL/6ByJ), rat (strains Sprague–Dawley, HiS, LoS, ACI and FH/Wjd strains), and human VTIR3 protein. The amino acid sequence was aligned with ClustalW and plotted with BOXSHADE. Black boxes indicate regions of identity, and gray boxes indicate conservative amino acid substitutions. In mice, higher or lower saccharin preferences are associated with a polymorphism at aa position 60 (isoleucine to threonine).