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. Author manuscript; available in PMC: 2006 Mar 14.

Published in final edited form as: FASEB J. 2005 Aug 12;19(12):1716–1718. doi: 10.1096/fj.04-2447fje

Figure 2. — Effects of cku-70 and cku-80 on adult hermaphrodite life span (days) at 25°C. A) For wild-type worms cku-70(RNAi) did not significantly change life span; mean life span ± SE was 14.1 ± 0.3 days (vector: n=136) and 15.0 ±0.3 [cku-70(RNAi): n=98], replicate experiments r = 2. B) rrf-3(pk1426) mean life span was slightly increased (P<0.01), 14.2 ± 0.3 (vector: n=96) and 15.5 ± 0.3 [cku-70(RNAi): n=98], r = 2. C) daf-2(e1370) mean life span was significantly increased (P<0.0001), 24.2 ± 1.1 (vector: n=97) and 32.6 ± 1.4 [cku-70(RNAi): n=64], r = 3. D) daf-16(m26) mean life span was significantly decreased (P<0.0001), 14.3 ± 0.3 (vector: n=93) and 12.8 ± 0.3 [cku-70(RNAi): n=97], r = 3.E) daf-16(m26);daf-2(e1370) mean life span was not significantly changed 13.5 ±0.4 (vector: n=51) and 14.5 ± 0.3 [cku-70(RNAi): n=61], r =2. F) In rrf-3(pk1426) cku-80(RNAi) had no effect on life span 14.2 ± 0.3 (vector: n=96) and 14.7 ±0.2 (cku-80(RNAi): n=98), r = 2. G) daf-2(e1370) mean life span was not affected by cku-80(RNAi), 22.1 ± 0.8 (vector: n=80) and 22.5 ± 10.7 [cku-80(RNAi): n=75], r = 2. H) glp-4(bn2);daf-2(e1370) life span was significantly increased (P<0.0001) compared with daf-2(e1370) alone, 33.2 ± 0.6 (vector: n=119) vs. 22.1 ± 0.8 (vector: n=80). glp-4(bn2);daf-2(e1370) life span was slightly increased (P<0.0001) by cku-70(RNAi), 37 ± 0.7 [cku-70(RNAi): n=117] but unchanged by cku-80(RNAi), 33.1 ± 0.7 [cku-80(RNAi): n=119], r = 2.

Figure 2. — Effects of cku-70 and cku-80 on adult hermaphrodite life span (days) at 25°C. A) For wild-type worms cku-70(RNAi) did not significantly change life span; mean life span ± SE was 14.1 ± 0.3 days (vector: n=136) and 15.0 ±0.3 [cku-70(RNAi): n=98], replicate experiments r = 2. B) rrf-3(pk1426) mean life span was slightly increased (P<0.01), 14.2 ± 0.3 (vector: n=96) and 15.5 ± 0.3 [cku-70(RNAi): n=98], r = 2. C) daf-2(e1370) mean life span was significantly increased (P<0.0001), 24.2 ± 1.1 (vector: n=97) and 32.6 ± 1.4 [cku-70(RNAi): n=64], r = 3. D) daf-16(m26) mean life span was significantly decreased (P<0.0001), 14.3 ± 0.3 (vector: n=93) and 12.8 ± 0.3 [cku-70(RNAi): n=97], r = 3.E) daf-16(m26);daf-2(e1370) mean life span was not significantly changed 13.5 ±0.4 (vector: n=51) and 14.5 ± 0.3 [cku-70(RNAi): n=61], r =2. F) In rrf-3(pk1426) cku-80(RNAi) had no effect on life span 14.2 ± 0.3 (vector: n=96) and 14.7 ±0.2 (cku-80(RNAi): n=98), r = 2. G) daf-2(e1370) mean life span was not affected by cku-80(RNAi), 22.1 ± 0.8 (vector: n=80) and 22.5 ± 10.7 [cku-80(RNAi): n=75], r = 2. H) glp-4(bn2);daf-2(e1370) life span was significantly increased (P<0.0001) compared with daf-2(e1370) alone, 33.2 ± 0.6 (vector: n=119) vs. 22.1 ± 0.8 (vector: n=80). glp-4(bn2);daf-2(e1370) life span was slightly increased (P<0.0001) by cku-70(RNAi), 37 ± 0.7 [cku-70(RNAi): n=117] but unchanged by cku-80(RNAi), 33.1 ± 0.7 [cku-80(RNAi): n=119], r = 2.