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. Author manuscript; available in PMC: 2006 Mar 14.
Published in final edited form as: Circ Res. 2005 Oct 27;97(11):e97–103. doi: 10.1161/01.RES.0000193595.03608.08

Figure 2.

Figure 2.

Activation of NF-κB in THP-1 cells. Sh. flexneri LPS at 50 ng/ml (solid bars) activated significantly only in the presence of both soluble CD14 (sCD14) and lipopolysaccharide binding protein (LBP). Natural human CRP at 100 μg/ml (open bars) had no activity alone or in the presence of both soluble CD14 (sCD14) and lipopolysaccharide binding protein (LBP). Recombinant bacterial CRP at 100 μg/ml (hatched bars) produced substantial activation in the presence of sCD14 and LBP, consistent with the presence of LPS. Similar results were obtained regardless of whether or not the recombinant CRP material was dialyzed before testing.