Figure 3.

Activation of NF-κB in TLR2 transfected HEK 293 cells. Expression of TLR2 (solid bars) enables dose dependent NF-κB activation by bacterial lipopeptides compared to absence of any effects in control cells transfected with vector alone (open bars). Neither natural nor recombinant CRP preparations produced any activation at all in either TLR transfected or control cells, suggesting absence of lipopeptide. Similar results were obtained regardless of whether or not the recombinant CRP material was dialyzed before testing.