Fig 3.

 D melanogaster sHsps inhibit heat-induced aggregation of luciferase. Luciferase (0.1 μM) was incubated at 42°C for 30 minutes either alone (opened lozenge) or in the presence of BSA (0.5 μM, open square), Hsp22 (A), Hsp23 (B), Hsp26 (C), or Hsp27 (D) at 2 different concentrations (0.1 μM: closed circle, 0.5 μM: closed triangle). Protein aggregation was determined by the light-scattering assay at 320 nm. Data are representative of 6 trials and are expressed as the mean ± standard deviation