Fig 3.

 p26-dependent thermotolerance in transfected mammalian cells. (A) E11′L cells and cells transfected with vector only were heat shocked for 0, 15, 30, 45, and 60 min at 46°C; incubated in T75 vented tissue culture flasks for 2 weeks; and stained with crystal violet. Nonshaded bar, E11′L; shaded bar, cells lacking p26. The data are the mean ± standard error of 3 independent experiments. (B) Crystal violet–stained flasks containing E11′L (left side) and control (right side) cells heat shocked for 30 min before incubation for 2 weeks. (C) Nomarski images of cells either containing (E11′L) or lacking (control) p26 after heat shock for 30 minutes. The bar in the lower right panel represents 50 μm, and all pictures are the same magnification