Fig 4.

 p26 reduces heat-induced apoptosis. E11′L and cells transfected with vector only were heat shocked at 46°C for the times indicated, incubated at 37°C for 24 hours, and stained with either annexin V-Alexa Fluor®488 conjugate (A) or Hoescht 33342 (B). Nonshaded bar, E11′L cells; shaded bar, cells lacking p26. The data are the mean ± standard error of 3 independent experiments. Cells heat shocked for 30 minutes were either double-stained with propidium iodide (red) and annexin V-Alexa Fluor®488 conjugate (green) (C, D) or stained with Hoescht 33342 (E). Arrows in panel E indicate apoptotic cell nuclei. Bar = 40 μm (D), which has the same magnification as (C). Bar = 15 μm (E)