Table 1. Helicobacter pylori strains used in this study.

Strain designation	Relevant genotype	hpyIIRM phenotype	Antibiotic resistance markera	Reference
6a	hpyIIRM+, cagA+, vacA s1m1	R+M+	None	(13)
6c	hpyIIRM–, cagA+, vacA s1m1	R–M–	None	(13)
CH4	hpyIIRM–, cagA+, vacA s1am1	R–M–	None	(5)
HPK1	hpyIIRM–, cagA+, vacA s1m1	R–M–	None	(8)
7767	hpyIIRM–, cagA+, vacA s2m2	R–M–	None	(8)
J166	hpyIIRM–, cagA+, vacA s1bm1	R–M–	None	(8)
J188	hpyIIRM+, cagA–, vacA s1m1	R–M–b	None	(8)
RA1 (fomerly 6aRK)	6a (hpyIIR::aphA)	R–M+c	Kan	(13)
RA2	6a (hpyIIR::aphA, rpoB15)	R–M+c	Kan, Rif	This study
RA3	6a (vacA::aphA)	R+M+	Kan	This study
RA4	6a (rpsL1, rrn16S1)	R+M+	Strep, Spec	This study
RA5	6c (rpsL1, rrn16S1)	R–M–	Strep, Spec	This study
RA6	J188 (rpsL1, rrn16S1)	R–M–b	Strep, Spec	This study
RA7	J166 (rpsL1, rrn16S1)	R–M–	Strep, Spec	This study

^aResistance to rifampin, streptomycin and spectinomycin due to point mutationsin rpoB (30), rpsL (31) and rrn16S (32), respectively. Resistance to kanamycin based on insertion of aphA in specified gene.

^bContains complete but inactive hpyIIRM cassette.

^chpyIIR^– due to interruption by aphA.