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Monitoring the endonuclease V digestion by agarose gel (1.6%) electrophoresis. M, DNA size standard (1 kb DNA ladder; Takara, Tokyo, Japan); lane 1, 0.2 mM dTTP/0 mM dUTP; lane 2, 0.15 mM dTTP/ 0.05 mM dUTP; lane 3, 0.1 mM dTTP/0.1 mM dUTP; lane 4, 0.05 mM dTTP/0.15 mM dUTP; lane 5, 0 mM dTTP/0.2 mM dUTP. See text for the other components of the reaction mixture.
