Fig. 3. Expression of M.grisea NTH1 during conidiogenesis, plant infection and in response to hyperosmotic stress. (A) RNA was extracted from a non-conidiating culture of M.grisea strain Guy11 (lane 1), a conidiating culture of Guy11 (lane 2) and a conidiating culture of nth1 mutant E508.R.6 (lane 3). RNA was also extracted from hyphal cultures of M.grisea grown in iso-osmotic media, CM (lanes 4 and 6), or subjected to acute hyperosmotic stress in CM + 0.5 M NaCl for 24 h (lanes 5 and 7). RNA was from strain Guy11 (lanes 4 and 5) or Δosm1 mutant JH73 (lanes 6 and 7). RNA gel blots were probed with NTH1. The 28S rRNA is shown as a loading standard. (B) Spatial expression of NTH1. Early conidiogenesis initiated from a foot cell in NTH1(p)::sGFP::Hph transformant T-4-11. (C) Conidial initials forming at the end of a conidiophore. (D) Mature conidiophore with three sympodially arrayed conidia. (E) Germ tube and appressorium on barley epidermis. (F) An invasive hypha entering an epidermal cell from the base of the same appressorium. Bar in all panels = 10 µm.