Fig. 2. Independent transcriptional control of kis and kid allows activation of the parD system in S.cerevisiae inhibiting cell proliferation conditionally. (A) Scheme depicting plasmid p303MKCKd. (B) Analysis of growth rates of budding yeast transformed with p303MKCKd or with pRS303 (control) in solid media supplemented with the indicated amounts (µM) of methionine and/or Cu²⁺. Samples were diluted in sterile water at the cell densities indicated and 10 µl of each dilution were used for each dot. Abbreviations: Met25, methionine repressible promoter; CUP1, copper inducible promoter; CYC1, CYC1 terminator.