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. 2004 Aug 16;166(4):465–471. doi: 10.1083/jcb.200404015

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — NuMA inhibition with LGN-N increases the length of spindle microtubules, in the presence or absence of centrosomes. (A–D) Tubulin distribution in an LGN-N–treated spindle (0.7 μM, added ∼3 min before image at t = 0) during live recordings (Video 5). (E) LGN-N was added to assembled spindles, samples were fixed after 8 or 15 min, spindle lengths were measured (mean ± SD, n = 15, two independent experiments), and normalized to the length of untreated spindles (40 μm). (F–H) Spindles assembled in the absence of centrosomes, around DNA beads (tubulin, red; DNA, blue). (F) Buffer control. (G) LGN-N–treated (2 μM, 8 min). (H) Higher magnified image of the region indicated in G. Times are in min:s. Bars, 10 μm.