Fig. 1.

Immunohistochemical analysis of rAAV vector expression. (a) rAAV expression cassette. ITR, inverted terminal repeats of AAV (120 bp), NSE (1,800 bp), and WPRE (650 bp); BGH, bovine growth hormone polyadenylation signal (250 bp). (b) HA immunoreactivity in human embryonic kidney 293 cells transduced with rAAV/CREB, rAAV/ICER, or rAAV/Empty vectors. (c and d) HA immunoreactivity within the rat brains that were transfected with rAAV/ICER, rAAV/Empty, or rAAV/CREB vectors. Animals were injected unilaterally into the hippocampus 4 weeks before immunohistochemistry analysis. Low-magnification (Upper) and high-magnification (Lower) images of the sections from different parts of the brain are shown. (e) Transgenic HA-ICER expression in aged animals (13 months after vector transfection). Anterior/posterior (AP) coordinates were measured from bregma. Arrows indicate the projection of the injection site with AP = −3.80 mm. (Scale bar, 1 mm.)