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. 2006 Mar 14;103(12):4705–4710. doi: 10.1073/pnas.0506137103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 5. — Postbehavioral in vivo transgene expression analysis. (a) Western blot analysis of hippocampus extracts derived from the rAAV/CREB-transfected animals with CREB or β-actin and GAPDH Abs. Animals expressing high or low levels of transgenic HA-CREB are indicated along with the control rAAV/Empty-transfected animal (C). Immunoblotting of increasing amounts of a hippocampus lysate with GAPDH Ab is also shown. (b) Correlation analysis of relative concentration of transgenic HA-CREB in rAAV/CREB-transfected rats with their individual combined latencies shown in the Barnes test. (c) Statistical analysis of combined latencies shown by all groups of animals in the Barnes task. (d) Comparative analysis of the latencies shown in the passive-avoidance task by rats expressing high or low levels of transgenic HA-CREB along with the other groups of animals. ■, high HA-CREB animals; •, low HA-CREB animals. Hatched bar, rAAV/CREB rats expressing low levels of HA-CREB; white bar, rAAV/CREB rats expressing high levels of HA-CREB; gray bar, rAAV/ICER rats; black bar, control animals.