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. 2006 Apr 3;116(4):916–928. doi: 10.1172/JCI27203

Figure 7. Zymosan induces TGF-β from spleen macrophages.

Figure 7

(A) C57BL/6 mice were injected i.v. with E. coli LPS (25 μg) or zymosan (25 μg) and bled at various time points; TGF-β induction in the serum was assayed by ELISA. *P < 0.05, zymosan- versus LPS-treated mice. (B) Biological activity of serum TGF-β was determined by stimulating total spleen cells with aliquots of serum and levels of phosphorylation of Smad-2 determined by Western blot. The addition of recombinant TGF-β was used as a positive control. (C) Spleen cells were isolated from the mice used in A at 6 or 10 hours and costained with TGF-β–TRITC and F4-80–FITC, then viewed by confocal microscopy. (D) Immunohistological analysis of TGF-β production. Splenic sections were prepared from spleens isolated at 1, 4, and 10 hours, stained with CD11b–FITC and TGF-β–TRITC, and analyzed by confocal microscopy. Arrows indicate double-positive cells. Original magnification, ×40.