Table 4.

Validation of microarray data for randomly selected genes by quantitative RT-PCR

	Real time RT-PCR (expression ratio)	Array (expression ratio)	Confirmed by real-time PCR
Kpnb3	−3.5	−2.83	Yes
Ivns1abp	−2.4	−2.51	Yes
Pfkfb3	−10.4	−2.42	Yes
Srcasm	−5.1	−3.55	Yes
Gadd45b	2.6	3.90	Yes
Stx11	2.2	4.75	Yes
Adam12a	1.2	1.14	Yes
Crybb3a	1.0	1.22	Yes
Cd9b	3.0	5.56	Yes

^aTwo genes with less then 2-fold change were tested for the false negative signals.

^bThe gene with more then 2-fold change in expression detected in two out of three experiments.

Note: “minus” sign in expression ratio column is used solely to indicate the decrease in expression level.