Fig. 1. Human γ-globin gene expression in transgenic mice carrying the –198^Aγ HPFH mutant. Lines A and B carry the construct µLCR –382^Aγ (–198 HPFH) while lines C, D and E carry the construct µLCR –382^Aγ ΔCACCC (–198 HPFH). RNase protection assays were done using the total RNAs from erythroid cells from various stages of development. d10 y/s, day 10 yolk sac; d12 y/s, day 12 yolk sac; d16 f/l, day 16 fetal liver; ad bl, adult blood. Hu γ indicates the protected fragment (170 bp) from exon 2 of the human γ mRNA. Mu ζ is the protected fragment (151 bp) from exon 1 of the murine ζ mRNA. Mu α is the protected fragment (128 bp) from exon 1 of the murine α mRNA. Notice that the –198 HPFH mutation increases the γ mRNA level.