Fig. 2. Proteins bound to the –198 C→T HPFH region are related to those bound to the γ gene CACCC box. A ³²P-labeled oligonucleotide encompassing the γCACCC box was used in the electrophoretic mobility shift assay. Nuclear extracts were prepared from MEL cells. Lane 1, the probe without competitor; lanes 2 and 3, competed with the cold probe; lanes 4 and 5, competed with an oligonucleotide corresponding to the sequence –185 to –210 of the γ gene promoter having a T→C mutation at position –198γ; lanes 6 and 7, competed with an oligonucleotide having the wild-type –185 to –210 sequence of the γ gene promoter. The concentration of the cold competitor was 30-fold higher than that of the hot probe in lanes 2, 4 and 6, and 50-fold higher in lanes 3, 5 and 7.