Table II. γ gene expressiona in the –175 HPFH transgenic mice.
| Construct | Line | Copy no. | Embryonic (primitive) erythropoiesis |
Definitive erythropoiesis |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 10b |
Day 12b |
Day 12b |
Day 16b |
Adult |
||||||
| Blood | Yolk sac | Blood | Yolk sac | Liver | Blood | Liver | Blood | |||
| µLCR –382Aγ | F | 3 | 6.0 ± 1.4 | 10.9 ± 2.7 | 13.7 ± 1.5 | 17.6 ± 3.0 | 15.8 ± 2.7 | 17.5 ± 2.8 | 31.7 ± 1.3 | 21.8 ± 4.5 |
| with –175 HPFH | G | 4 | 10.2 ± 2.3 | 21.2 ± 5.0 | 22.6 ± 2.4 | 49.6 ± 14.5 | 46.1 ± 10.8 | 49.8 ± 9.9 | 54.6 ± 10.8 | 25.9 ± 2.2 |
| H | 10 | 1.8 ± 0.7 | 3.2 ± 0.5 | 5.7 ± 2.0 | 8.5 ± 2.7 | 20.0 ± 4.8 | 15.1 ± 6.0 | 24.8 ± 4.2 | 16.9 ± 3.9 | |
| I | 6 | 10.2 ± 3.9 | 15.5 ± 2.6 | 27.9 ± 5.5 | 25.9 ± 3.8 | 58.2 ± 4.8 | 48.7 ± 1.0 | 45.3 ± 5.9 | 25.3 ± 1.1 | |
| J | 8 | 1.2 ± 0.4 | 1.8 ± 0.3 | 2.0 ± 1.4 | 3.9 ± 1.7 | 9.6 ± 2.7 | 5.0 ± 1.1 | 5.5 ± 0.1 | 4.9 ± 2.7 | |
| mean | 5.8 ± 4.4 | 10.5 ± 8.2 | 14.8 ± 10.9 | 21.1 ± 18.0 | 29.9 ± 21.0 | 27.2 ± 20.7 | 32.4 ± 19.0 | 19.1 ± 8.6 | ||
| µLCR –382Aγ ΔCACCC | K | 11 | 7.0 ± 1.2 | 9.3 ± 1.8 | 16.2 ± 3.5 | 23.9 ± 3.1 | 14.0 ± 2.1 | 7.5 ± 2.0 | 7.3 ± 0.6 | 4.7 ± 0.7 |
| with –175 HPFH | L | 2 | 0.44 ± 0.1 | 0.75 ± 0.1 | 0.44 ± 0.1 | 1.1 ± 0.2 | 0.70 ± 0.3 | 0.75 ± 0.2 | 0.61 ± 0.4 | 0.70 ± 0.3 |
| M | 58 | 2.0 ± 0.3 | 3.0 ± 0.3 | 4.9 ± 1.9 | 29.7 ± 8.0 | 9.5 ± 1.6 | 7.4 ± 2.0 | 6.7 ± 0.7 | 2.7 ± 0.8 | |
| N | 58 | 1.5 ± 0.2 | 3.8 ± 0.1 | 3.4 ± 0.2 | 20.2 ± 4.2 | 7.6 ± 0.6 | 2.8 ± 0.1 | 3.9 ± 1.0 | 1.1 ± 0.2 | |
| O | 3 | 1.1 ± 0.2 | 2.3 ± 0.2 | 3.4 ± 1.2 | 5.5 ± 1.8 | 1.1 ± 0.1 | 0.47 ± 0.1 | 0.47 ± 0.1 | 0.22 ± 0.1 | |
| mean | 2.4 ± 2.6 | 3.8 ± 3.3 | 5.7 ± 6.1 | 16.1 ± 12.2 | 6.6 ± 5.9 | 3.8 ± 3.5 | 3.8 ± 3.2 | 1.9 ± 1.8 | ||
| µLCR –382Aγ | mean | 5.7 ± 6.3 | 5.2 ± 3.3 | 10.4 ± 9.5 | 21.0 ± 7.4 | 11.8 ± 2.8 | 13.4 ± 4.7 | 15.7 ± 4.8 | 16.5 ± 4.5 | |
| µLCR –382Aγ ΔCACCC | mean | 4.2 ± 0.6 | 6.1 ± 1.7 | 8.9 ± 4.3 | 13.9 ± 3.9 | 5.2 ± 3.0 | 2.9 ± 1.3 | 2.6 ± 0.9 | 0.9 ± 0.6 | |
aHuman γ mRNA is expressed as a percentage of murine α mRNA per copy. Mean values are shown in bold. Data in µLCR –382Aγ control mice are from Stamatoyannopoulos et al. (1993).
bDays post-coitus.