Fig. 4. Effect of GSK-3β overexpression on β-catenin levels. (A) Representative western blot of total cellular and nuclear preparations from the cortex (Cx) and hippocampus (Hipp) of wild-type (Wt) or Tet/GSK-3β (Tg) mice probed with anti-β-catenin antibody. β-tubulin and U2snRNP blots are shown as controls of total and nuclear protein loading. (B) Quantification of patches of β-catenin in the nucleus of dentate gyrus granule cells from wild-type and Tet/GSK-3β mice as detected by immunoelectron microscopy. (C) Electron microscopy photomicrograph showing a dentate gyrus neuron from a wild-type mouse with several patches of β-catenin reaction product (arrowheads). NU, nucleolus. (D) Electron microscopy photomicrograph showing a dentate gyrus neuron from a Tet/GSK-3β mouse. Asterisks, astrocitic process. No heavy metal staining was performed. Scale bars in (C) and (D) correspond to 0.1 µm.