Fig. 6. Disruption of CHC22 TGN localization by low temperature. HeLa cells, infected with pLNCXCHC22 to obtain low-level enhanced expression of CHC22, were either left untreated (A–C) or incubated at 20°C for 1 h (D–F) before being subjected to digitonin wash to remove cytosol, and then processed for immunofluorescence. CHC22 (red) was detected with anti-CHC22 antiserum followed by LRSC-conjugated goat anti-rabbit IgG (A and D). AP1 (green) was stained with MAb 100/3 followed by FITC-conjugated goat anti-mouse IgG (B and E). The columns represent the same images viewed with different filters and the bottom panels are merged images.