Fig. 3. Stable segregation of YRp7FR in the presence of EBNA1 and hEBP2. Plasmid loss assays were conducted over 11 generations in KY320 (left panel) or W303-1A (right panel) yeast strains containing a segregation plasmid, a URA3 expression plasmid that contains or lacks the EBNA1 gene, and a LEU2 expression plasmid that contains or lacks the hEBP2 gene. Dilutions of the cultures were then grown on SC-Ura,Leu (no selection for test plasmid) or SC-Ura,Leu,Trp (selection for test plasmid) plates.